Rapid Detection and Sequence-Specific Differentiation of Extended-Spectrum β-Lactamase GES-2 from Pseudomonas aeruginosa by Use of a Real-Time PCR Assay

摘要

The LightCycler was compared to nested PCR for the detection of blaGES/IBC genes from 100 Pseudomonas aeruginosa clinical isolates. The real-time PCR assay detected a blaGES/IBC gene product from 83 isolates, exhibiting a sensitivity and specificity of 94.3 and 100% respectively, compared to nested PCR and DNA sequencing.